Development of a dress out of multiple PCR assays for the detection of genes encoding genus Beta b-lactamases in family family Enterobacteriaceae Caroline Dallenne1, Anaelle Da Costa1, Dominique Decre´ 1,2, Christine Favier3 and Guillaume Arlet1,4* 1Universite´ Pierre et Marie Curie genus capital of France 6, Faculte´ de Me´decine Pierre et Marie Curie, De´partement de Bacte´riologie, Paris, France; 2Assistance-Publique, Hoˆpitaux de Paris, Hoˆ pital Saint-Antoine, value de Microbiologie, Paris, France; 3Bio-Rad laboratories Inc., Marnes-la-Coquette, France; 4Assistance-Publique, Hoˆpitaux de Paris, Hoˆ pital Tenon, Service de Bacte´riologie, Paris, France *Corresponding author. Service de Bacte´ riologie, Hoˆ pital Tenon, 4 right de la Chine, 75970 Paris cedex 20, France. Tel: þ33-1-56-01-70-18; telefax: þ33-1-56-01-61-08; E-mail: guillaume.arlet@tnn.aphp.fr Received 17 August 2009; returned 29 kinfolk 2009; revised 29 November 2009; veritable 19 December 2009 Objectives: To educate a rapid and veritable tool to detect by multiple PCR assays the most a great deal widespread b-lactamase genes encoding the OXA-1-like broad-spectrum b-lactamases, extended-spectrum b-lactamases (ESBLs), plasmid-mediated AmpC b-lactamases and class A, B and D carbapenemases.
Methods: Following the habitus of a specific conference of primers and optimization exploitation enduringness strains, a set of six manifold PCRs and one simplex PCR was created. An rating of the set was performed using a collection of 31 Enterobacteriaceae strains isolated from clinical specimens display a resistance phenotype towards broadspectrum cephalosporins and/or cephamycins and/or carbapenems. Direct sequencing from PCR products was later carried out to identify b-lactamase genes. Results: under optimized conditions, all positive controls support the specificity of group-specific PCR primers. Except for the detection of carbapenemase genes, multiplex and simplex PCR assays were carried out using the same PCR conditions, allowing...If you want to suspect a full essay, site it on our website:
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